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D9-Design and Evaluation of Sacran/Cyclodextrin Hydrogen Films for Wound Dressing Materials (Nasrul Wathoni; Motoyama K.; Higashi T.; Okajima M.; Kaneko T.; Arima H.)

A wound dressing is one of theessentialapproaches to prevent further harm tocutaneous wounds as well asto promote wound healing. Therefore, to ...

  • CodeCallNoLokasiKetersediaan
    FFUP20170064D9Tersedia
  • Perpustakaan
    Fakultas Farmasi
    Judul Seri
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    No. Panggil
    D9
    Penerbit : .,
    Deskripsi Fisik
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    Bahasa
    English
    ISBN/ISSN
    -
    Klasifikasi
    NONE
    Tipe Isi
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    Tipe Media
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    Tipe Pembawa
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    Edisi
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    Info Detil Spesifik
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    Pernyataan Tanggungjawab
  • A wound dressing is one of theessentialapproaches to prevent further harm tocutaneous wounds as well asto promote wound healing. Therefore, to achieve an ideal wound healing, the development of advanced dressing materials is necessary. Sacran, a novel megamolecular polysaccharide derived from the cyanobacterium Apanothece sacrum, has very high molecular weight that exceeds 107 g/mol and water-superabsorbent capacity (Fig. 1). Recently, we demonstrated that sacran provides anti-inflammatory activity by ameliorating the skin barrier function in patients with atopic dermatitis.In this study, to develop and characterize sacran hydrogel films (Sac-HGFs) for wound dressing materials, we prepared a physically crosslinked-Sac-HGFs, and evaluated their physicochemical properties, cytotoxicity, skin hydration and wound healing ability. Additionally, to ameliorate thephysicochemical properties of the Sac-HGFs, we nextprepared physically crosslinked Sac-HGFs containing cyclodextrins (CyDs), and examined the potentials as wound healing materials. Finally, we encapsulatedcurcumin, asa model drug for wound healing,into Sac-HGFs by complexation with 2-hydroxypropyl-γ-cyclodextrin (HP--CyD), and investigated their potential for wound dressing application.
    The physicochemical characterization of aphysically crosslinked-Sac-HGFshowed that the swollen ratio of theSac-HGFin water at 24 h was 19-fold, compared to initial weight. Meanwhile, thesodium alginate HGF was completely broken apart after rehydration.Moreover, the Sac-HGF did not show any cytotoxicity in NIH3T3 cells, a murine fibroblast cell line.The in vivo skin hydration study revealed that the Sac-HGF significantly increased the moisture content on hairless mice skin. In addition, the Sac-HGF, which was applied on wound site, considerably improved wound healing ability, compared to control (non-treated), probably due to not only the moisturizing effect but also the anti-inflammatory effect of sacran.
    The sacran/-CyD hydrogel film (Sac/-CyD-HGF) and sacran/-CyD HGF (Sac/-CyD-HGF), but not sacran/-CyD HGF (Sac/-CyD-HGF), were well prepared without surface roughness. Powder X-ray diffraction (XRD) pattern of the Sac/-CyD-HGF showed a totally amorphous state, compared to thatof the Sac/-CyD-HGF. Furthermore, the addition of -CyD to Sac-HGF significantly increased the swelling ratio, porosity, and moisture content, compared to those of the Sac-HGFs without CyDs. The Sac/-CyD-HGFs were not cytotoxicin NIH3T3 cells. Notably, the Sac/-CyD-HGFs significantly improved wound healing in mice, compared to that achieved with the Sac-HGF without -CyD.
    We successfully prepared curcumin/HP--CyD(Cur/HP--CyD) complex in Sac-HGFwithout surface roughness. In addition, the results from powder XRD patterns and differential scanning calorimetry thermographs showed the amorphous form intheCur/HP--CyD complex in Sac-HGF. In contrast, the curcumin in Sac-HGF and curcumin/HP--CyD physical mixture (Cur/HP--CyD PMX) in Sac-HGF formed inhomogeneousHGFs due to the crystallization of curcumin. Furthermore, HP--CyD had an important role to increase the elastic modulus of the Sac-HGF withhigh re-swelling ability. The Cur/HP--CyD complex in Sac-HGF maintained antioxidant properties of curcumin. From in vitro drug release studies, the curcumin was gradually released from the HP--CyD complex in Sac-HGF. Notably, the Cur/HP--CyD complex in Sac-HGF provided the highest wound healing ability in hairless mice.
    Based on the results mentioned above, it can be concluded that Sac-HGF has the potential properties for wound dressing application, due to not only the moisturizing effect but also the anti-inflammatory effect of sacran. Additionally, the characterization studies ofSac/CyD-HGFs suggested that the formation of homogenous and amorphous Sac/CyD-HGFs was affected by water solubility of CyDs. Notably, the presence of -CyD in Sac-HGF significantly improved the physicochemical properties of Sac-HGF. In addition, the Sac/-CyD-HGFmarkedly improved wound healing in mice, compared to that achieved with the Sac-HGF without -CyD.Furthermore, the Sac-HGF has the potential to deliver water soluble complex of curcumin/HP--CyD at the wound site and promote the wound healing ability. These findings may be useful information for preparation of wound dressing materials using sacran, CyDs and drugs.





    Fig.1. Partial Structural Formula of Sacran

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