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Manuscript  

Ancient Human Tooth DNA Extraction Method: A Rapid Review

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• Code	CallNo	Lokasi	Ketersediaan
  OB1 - 163	163 611 Wiw A	Jatinangor (Oral Biologi)	Tersedia namun tidak untuk dipinjamkan - No Loan

• Perpustakaan	Fakultas Kedokteran Gigi
  Judul Seri	-
  No. Panggil	611 Wiw A
  Penerbit	FKG Unpad : FKG UNPAD JATINANGOR., 2021
  Deskripsi Fisik	-
  Bahasa	English
  ISBN/ISSN	160110180028
  Klasifikasi	611
  Tipe Isi	-
  Tipe Media	-
  Tipe Pembawa	-
  Edisi	-
  Subyek	DNA Extraction Methods for Ancient Teeth Human Ancient DNA, Phenol-chloroform Silica based
  Info Detil Spesifik	-
  Pernyataan Tanggungjawab	Ajeng Pramesti Putri Wiwitan (Author)

• ntroduction: Research on DNA analysis of ancient human teeth is still not optimal due to the lack of the DNA extraction process. Although teeth are the most resistant part of the body to protect the organic and inorganic materials, problems in extracting ancient human DNA still occur which results in short DNA molecules which can complicate the DNA extracting process. This research was conducted to map the success of ancient human teeth DNA extraction using a variety of substrates that were successfully carried out by previous researchers Methods: This research was conducted using literature review method through a rapid review approach by collecting articles through an electronic search engine such as PubMed, EbscoHost, Sicence Direct, Research Gate, and Cambridge Core that matched the inclusion and exclusion criteria. Results: From 6 articles that included, the ancient human DNA extraction method with silica-based materials was more often used by researcher, especially for short DNA base pairs. Conclusion: Extracting ancient human tooth DNA using a silicabased protocol with poor preservation can produced historical age, mtDNA, and microbiota in dental calculus in fully demineralized samples. Meanwhile, extracting DNA from ancient human teeth using phenol-chloroform can describe eyes and hair colour of ancient humans.

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